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Chen, Mei-Yu
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  • Education: M.D., National Yang-Ming Medical College ; Ph.D., BCMB program, Johns Hopkins University School of Medicine
  • Office: R602, 6F, Tradition Medicine Building
  • Phone: 886-2-28267269
  • Email:  meychen@ym.edu.tw This e-mail address is being protected from spambots. You need JavaScript enabled to view it
  • Personal Web Site
  • Research--Molecular mechanism of amoeboid chemotaxis and cancer invasion ; Mechanism of TOR signaling
  • Mechanism and regulation of amoeboid chemotaxis--Amoeboid chemotaxis is of fundamental importance in cell-mediated immunity as well as tumor invasion and metastasis. Dictyostelium provides an excellent model system for studying chemotaxis since this organism spends most of its life cycle as chemotactic amoeboid cells and chemotaxis plays a pivotal role during its starvation-induced developmental program. We perform mutagenesis in Dictyostelium, screen for mutants with aberrant chemotactic behaviors, and from the collected mutants, identify and clone the genes responsible for the phenotype. Further analyses of the identified novel genes and their homologs in mammals should provide more insights into the molecular mechanism and regulation of eukaryotic chemotaxis.
  • Functions of Pianissimo homologs in TOR signaling pathway-- Pianissimo (Pia) was first identified in Dictyostelium as a novel cytosolic protein involved in cAMP signaling pathway. Pia-like proteins exist in a diverse organisms ranging from yeast to mammals. Using a combination of molecular and genetic methods, we have found that the Saccharomyces cerevisiae homolog (Tsc11p or Avo3p) is involved in modulating cell integrity and interacts functionally with the target of rapamycin (Tor2p) signaling pathway. In yeast, Tor2p controls cell growth and regulates the cell cycle-dependent organization of actin cytoskeleton. Further studies are underway to elucidate the role of Tsc11p in these signaling pathways.
  • Publications
    • Chen, M.Y., Insall, R. H. and Devreotes, P. N. (1996) Signaling through chemoattractant receptors in Dictyostelium. Trends Genet. 12,52-57.

    • Chen, M.Y., Long, Y. and Devreotes, P. N. (1997) A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the twelve transmembrane domain adenylyl cyclase in Dictyostelium. Genes Dev. 11, 3218-3231.

    • Ho, H.L., Shiau, Y.S., and Chen, M.Y. (2005) The Saccharomyces cerevisiae TSC11/AVO3 gene participates in regulating cell integrity and functionally interacts with components of the Tor2 complex. Curr. Genet. 47(5), 273-288.

    • Pang, T.-L., Wu, C.-J., Chen, P.-A., Weng, Y.-L., and Chen, M.-Y. (2007) Dictyostelium gnt15 encodes a protein with similarity to LARGE and plays an essential role in development. Biochem. Biophys. Res. Commun. 360, 83-89.

    • Hwa, K.-Y., T.-L. Pang, and Chen, M.-Y. (2007) Classification of LARGE-like GlcNAc-transferases of Dictyostelium discoideum by phylogenetic analysis. IEEE CS Proceedings FBIT2007, 386-391.

    • Ho, H.-L., Lee, H.-Y., Liao, H.-C., and Chen, M.-Y. (2008) Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Euk. Cell 7, 1328-1343.

    • Tang, L., Franca-Koh, J., Xiong, Y., Chen, M.-Y., Long, Y., Bickford, R. M., Knecht, D. A., Iglesias, P. A., and Devreotes, P. N. (2008) tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis. Genes Dev. 22, 2278-2290.

      Hwa, K.-Y., Lin, W.-M, Lee, C.-P., and Chen, M.-Y. (2009) Knowledge management on the novel LARGE-like GlcNAc-transferase protein family. IEEE CS Proceedings CCIS 57, 141-145.

    • Chou, T.-Y., Chen, W.-C., Lee, A.-C., Hung, S.-M., Shih, N.-Y., and Chen, M.-Y. (2009) Clusterin silencing in human lung adenocarcinoma cells induces a mesenchymal-to-epithelial transition through modulating the ERK/Slug pathway. Cell. Signal. 21, 704-711.

    • Chen, C.-Y., Yang, K.-Y., Chen, M.-Y., Chen, H.-Y., Lin, M.-T., Lee, Y.-C., Perng, R.-P., Hsieh, S.-L., Yang, P.-C., and Chou, T.-Y. (2009) Decoy receptor 3 levels in peripheral blood predict outcomes of acute respiratory distress syndrome. Am. J. Respir. Crit. Care Med. 180, 751-760.

    • Pang, T.-L., Chen, F.-C., Weng, Y.-L., Liao, H.-C., Yi, Y.-H., Ho, C.-L., Lin, C.-H., and Chen, M.-Y. (2010) Costars, a Dictyostelium protein similar to the carboxyl part of STARS, regulates actin cytoskeleton and motility. (in revision)

     
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